2D-immunoblotting analysis of Sporothrix schenckii cell wall
نویسندگان
چکیده
منابع مشابه
2D-immunoblotting analysis of Sporothrix schenckii cell wall.
We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glyco...
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Systemic sporotrichosis is an emerging infection potentially fatal for immunocompromised patients. Adhesion to extracellular matrix proteins is thought to play a crucial role in invasive fungal diseases. Here we report studies of the adhesion of Sporothrix schenckii to the extracellular protein fibronectin (Fn). Both yeast cells and conidia of S. schenckii were able to adhere to Fn as detected ...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2011
ISSN: 0074-0276
DOI: 10.1590/s0074-02762011000200021